These ischemia-induced alterations include increased retinal cell death (i.e., ARPE-19), a reduction in ERG b-wave amplitude, decreased fluorogold-labeled RGC density, and upregulation of neovascular/angiogenic/inflammatory, including β-catenin, HIF-1α, VEGF, and Ang-2. The gene discussed is HIF1A; the disease is ischemia.