Among the isolated compounds, Lupeol demonstrated the most potent bioactivity, exhibiting marked cytotoxicity against DU-145 prostate and HL-60 leukemia cell lines in both MTT and SRB assays, alongside strong NF-κB inhibition, indicating its chemo-preventive and apoptosis-inducing potential. The gene discussed is NFKB1; the disease is leukemia.