Notably, although prior studies identified karyopherin subunit alpha 2 (KPNA2, in breast cancer metastasis) and ribosomal protein S16 (RPS16, in hepatocellular carcinoma) as USP1 substrates, these proteins were not detected as USP1 targets in the current CCA model. Here, KPNA2 is linked to hepatocellular carcinoma.