N-glycosylation proceeds via the OST–ER trimming–Golgi extension cascade; tunicamycin blockade of N-glycan transfer slows MUC2 dimer/assembly and impairs secretion, and misfolded Muc2 in Winnie/Eeyore models links folding defects to spontaneous colitis [18,58]. This evidence concerns the gene MUC2 and colitis.