Conversely, significantly higher usages of TRBV6-7, TRBV16, TRBV23-1, and TRBV30 were observed in CRC patients compared to healthy donors (Figure 2G, FDR-corrected Mann–Whitney U test, * p < 0.05, ** p < 0.01, *** p < 0.001). Here, TRBV16 is linked to colorectal carcinoma.