Furthermore, significantly higher usages of TRBV1, TRBV4-3, TRBV5-2, TRBV5-3, TRBV5-7, TRBV6-1, TRBV6-8, TRBV6-9, TRBV7-1, TRBV7-3, TRBV7-5, TRBV12-1, TRBV12-2, TRBV21-1, and TRBV22-1 were observed in healthy donors compared to CRC patients among the functional human Vβ genes. Here, TRBV22-1 is linked to colorectal carcinoma.