As observed in Figure 7a, Hdac4 presented an inverse expression profile to that observed for its regulator myomiR, miR-1-3p (Figure 6a); as it is significantly downregulated in response to NAC and also presented a trend to be less expressed in the NAC + CIS group when compared to CIS-treated rats, suggesting that miR-1-3p possibly promotes myogenesis at least in part by negatively regulating Hdac4 expression in NAC presence. This evidence concerns the gene HDAC4 and in situ carcinoma.