Furthermore, 4 hub genes associated with senescence in IDD were screened by intersecting the results of LASSO and randomForest algorithms (Fig. 4A), comprising BID, KANK2, SMIM3, and ZEB2. The diagnostic performance of 4 hub genes for IDD in GSE70362 dataset was evaluated by ROC curve, and the hub genes showed higher specificity and sensitivity, with BID AUC = 0.87, KANK2 AUC = 0.86, SMIM3 AUC = 0.87, and ZEB2 AUC = 0.89 (Fig. 4B). This evidence concerns the gene KANK2 and intervertebral disk degenerative disorder.