As several subunits from the menin-MLL1 complex were identified as negative regulators of HCC survival in both 2D and 3D CRISPR-Cas9 screens and the function of this complex as a unit has not been previously studied in HCC, we decided to focus on deciphering its role in regulating transcription and chromatin in HCC. This evidence concerns the gene KMT2A and hepatocellular carcinoma.