We then investigated whether menin loss and inhibition affected pathways associated with cancer using the MSigDB database HALLMARK (H) and Oncogenic Signatures sets (C6).32 This revealed a consistent upregulation across both cell lines of epithelial-mesenchymal transition (EMT), KRAS-associated pathways, and estrogen response genes (Figures 3E, S3C, and S3D). This evidence concerns the gene KRAS and cancer.