To evaluate pathways regulated by menin-MLL1 in HCC, we performed ontology analysis using the Genomic Regions Annotation Tool (GREAT31) of gene promoters directly bound by both menin and MLL1 proteins and identified enrichment for the tumor necrosis factor alpha (TNF-α), transforming growth factor β (TGF-β), MTORC1, PI3K/AKT, and Hedgehog signaling pathways (Figure 2M; Table S4). This evidence concerns the gene AKT1 and hepatocellular carcinoma.