FAT1 and Glucose intolerance: Based on the increased weight gain observed in S. aurata subjected to regular administration of chitosan-TPP-DNA nanoparticles to induce long-term sustained expression of C. elegans fat-1 and fat-2 [14], and considering that fat-1 transgenesis prevents liver steatosis, glucose intolerance, and insulin resistance while exerting protective vascular effects through reduced inflammation [10,18,19,20,43], we hypothesized that elevated n-3 LC-PUFA levels and a decreased n-6/n-3 fatty acid ratio may contribute to improved fish health and enhanced immune status.