In vitro: at the maximum concentration of MNPs (250 μg/mL), a reduced cell viability of less than 10% occurred. MG were incubated with interleukin 4 (IL-4)-pretreated M2 macrophages. An enhancement of M1 macrophages number concomitantly with an important decrease in M2 macrophages population was noticedIn vivo: the MG group exhibited the lowest tumor fluorescence intensity and the smallest tumor volume. The mice’s body weight was maintained 10 days after surgery, indicating no signs of toxicity. The histopathological analysis revealed an increase in M1 macrophages compared to the M2 state. The gene discussed is IL4; the disease is neoplasm.