Moreover, transgenic mice overexpressing calpastatin or CAST (natural negative regulators of calpains) subjected to an anterior coronary artery ligation-induced myocardial infarction (MI) exhibit higher mortality at six weeks than wild-type (WT) mice with a high incidence of cardiac rupture during the first week post-MI [194]. The gene discussed is CAST; the disease is myocardial infarction.