NeoPAIR-T, the overall workflow of which is illustrated in Figure 3, enables multiplexed identification of bona fide neoantigen–TCR pairs by systematically integrating transcriptome-guided prediction of tumor-specific TCRs with MHC-binding–based prediction of candidate neoantigens, their efficient expression in autologous APCs, and functional screening using reporter T cells. This evidence concerns the gene HLA-C and neoplasm.