AIF1 and central nervous system cancer: Taken together, we have demonstrated that after H-FIRE ablation of glioma, cells release populations of extracellular vesicles containing sTDEVs, which have significantly altered proteomic payloads, increased brain- and endothelial cell tropism, and correlate with increased Iba1+ cell infiltration compared to sTDEVs released by 0 V/cm-treated glioma cells.