Confocal microscopy revealed that animals receiving disruptive sTDEVs, those released from 3000 V/cm H-FIRE-treated glioma cells, had significantly increased numbers of Iba1+ cells per field of view in the brain compared to animals that received non-disruptive sTDEVs released by 0 V/cm-treated glioma cells (Figure 7A,B). This evidence concerns the gene AIF1 and central nervous system cancer.