We quantified the number of Iba1+ cells per field of view in animals that received sTDEVs released by 0 V/cm- and H-FIRE-treated glioma cells (n = 3 and n = 2, respectively) 24 h post-administration to determine whether sTDEVs released after H-FIRE ablation of glioma, which are maintained in the brain, may contribute to the local immune response via macrophage recruitment. The gene discussed is AIF1; the disease is glioma.