For instance, a rat model treated with both letrozole and a high-fat diet displayed anovulatory cycles, polycystic ovaries, elevated testosterone, glucose intolerance and dyslipidemia, along with impaired insulin signaling, as evidenced by reduced phosphorylation of INSR, IRS, PI3K and AKT in classic insulin-target tissues [180]. Here, AKT1 is linked to polycystic ovary syndrome.