BC patients demonstrate significantly elevated phosphorylated protein levels of select EV proteins—including Ral GTPase-activating protein subunit alpha-2 (RALGAPA2), cGMP-dependent protein kinase1 (PKG1), tight junction protein 2 (TJP2), and nuclear transcription factor, X box-binding protein 1 (NFX1) [448]. This evidence concerns the gene TJP2 and breast cancer.