To investigate altered signaling due to different KRAS mutations, immunoprecipitation followed by mass spectrometry-based proteomics was employed to characterize the differential interactomes of WT and mutant KRAS proteins expressed in HKe-3 CRC cells, derivatives of HCT116 cells in which the endogenous KRAS G13D allele has been knocked out [31]. Here, KRAS is linked to colorectal carcinoma.