For example, modified-release insulin-loaded liposomes coated with poloxamer F188 and PEG500 were fabricated via the heating method with particle sizes of 76.6–155.0 nm and encapsulation efficiencies of 17.61–76.08% administered via subcutaneous (SC) or oral (PO) routes in streptozotocin-induced type 1 diabetic Wistar rats [4]. The gene discussed is INS; the disease is type 1 diabetes mellitus.