SYTL5 and osteosarcoma: Using the osteosarcoma-derived U2OS cell line, here we confirm that SYTL5 interacts with RAB27A and show that it localises to mitochondria and small, highly mobile vesicles interacting with the mitochondrial network, as well as to endolysosomal and endocytic compartments, indicating a role of SYTL5 in cellular membrane trafficking events.