Acute colitis was induced in wild-type (WT), TLR2 knockout (KO), TLR4 KO, and TLR9 KO mice by administrating the mouse pathogen C. rodentium, then colitis severity was evaluated based on body weight changes, pathology, and mucosal cytokine gene expression at two weeks after infection (acute phase). This evidence concerns the gene TLR4 and colitis.