Fentanyl-treated mice showed significantly greater Iba1+ coverage at plaques compared to saline controls in both cortex (Figure 3B; AD-Sal = 38.67±1.97, AD-ROU = 66.73±1.59, p = 1.6E-6) and hippocampus (AD-Sal = 22.16±1.41, AD-ROU = 61.33±1.13, p = 3.7E-8). This evidence concerns the gene AIF1 and Alzheimer disease.