Given that nuclear translocation of PFKFB3 was mainly through the importin α/β pathway,38 we employed importazole, the importin β inhibitor, to intervene the irradiated HCC cells on high‐stiffness substrate, and immunofluorescence staining and nuclear protein analysis all demonstrated that importazole intervention partially attenuated PFKFB3 nuclear translocation in the irradiated cells on high‐stiffness substrate (Figure 5D; Figure S6C). This evidence concerns the gene PFKFB3 and hepatocellular carcinoma.