We further examined the changes of proliferation indexes in HCC cells with PFKFB3 knockdown under high‐stiffness stimulation, and found that PFKFB3 suppression significantly downregulated the expressions of Ki67 (Figure 3G) and proliferation‐associated proteins PCNA, and CCND1 (Figure 3H) in HCC cells, confirming a positive correlation between PFKFB3 expression and cell proliferation. Here, CCND1 is linked to hepatocellular carcinoma.