Subsequently, regardless of taking PFKFB3 as a bait protein to capture Ku70 or taking Ku70 as a bait protein to capture PFKFB3 in nuclear protein of irradiated HCC cells, we all verified an endogenous binding between PFKFB3, Ku70 and x‐ray repair cross‐complementing protein 4 (XRCC4) in the nucleus of irradiated HCC cells (Figure 6B; Figure S7A), suggesting that PFKFB3 may participates in NHEJ. Here, PFKFB3 is linked to hepatocellular carcinoma.