Profiling key glycolytic enzymes in HCC cells under different stiffness stimulation revealed a striking, selective upregulation of PFKFB3, but other critical glycolytic enzymes, including phosphofructokinase platelet (PFKP), hexokinase 2 (HK2), lactate dehydrogenase A (LDHA) and pyruvate kinases M2 (PKM2), remained unaltered in expression (Figure 3B). Here, PFKFB3 is linked to hepatocellular carcinoma.