Additionally, PFKFB3 expression was significantly higher in human HCC tissues classified as high‐stiffness (COL1High/LOXHigh, n = 32) versus low‐stiffness (COL1Low/LOXLow, n = 33) (Figure 3I), and its pathological significance analysis also supported a positive association between high PFKFB3 expression and increased tumour size (Table 1). This evidence concerns the gene PFKFB3 and hepatocellular carcinoma.