Western blotting showed that the protein levels of NF-α1/CPE and NF-α1/CPE-E342Q were significantly increased in 3 × Tg-AD mice after AAV-NF-α1/CPE and AAV-NF-α1/CPE-E342Q treatment, compared to those treated with GFP (Fig. 2a). This evidence concerns the gene CPE and Alzheimer disease.