This was further validated by Western blotting, showing that treatment with AAV-NF-α1/CPE or AAV-NF-α1/CPE-E342Q significantly decreased Trim28 and Snx4 levels in 3 × Tg-AD mice compared with 3 × Tg + GFP mice (Fig. 5b, c). This evidence concerns the gene SNX4 and Alzheimer disease.