TNF and neoplasm: These splenocytes of the cured A20 model stimulated with A20 cells secreted higher concentration of IFN-γ and TNFα than did those stimulated with 4T1, Panc02 and CT26 cells, whereas splenocytes of treatment-naive mice showed no detectable response to any stimulation (Fig. 5C), indicating that the intratumoral treatment with OVV-mNbTIM3 generated tumor-specific memory in the splenocytes.