Total distance traveld and time spent in the center area during the open-field test did not differ between genotypes (total distance: floxed-GR, 46.8 ± 2.52 m, N = 9; GR-ThKO, 52.1 ± 3.96 m, N = 10; p = 0.285 by Student’s t-test; center time: floxed-GR, 72.9 ± 5.65 s, N = 9; GR-ThKO, 89.9 ± 9.20 s, N = 10; p = 0.143 by Student’s t-test; Supplementary Fig. 9a–c), suggesting that locomotor activity and anxiety-like behavior were not alterd in GR-ThKO mice. The gene discussed is NR3C1; the disease is Anxiety.