In the HFFD-induced mouse model of MASH, the observed bioinformatics data were consistent with in vivo findings: HFFD-fed mice showed reduced hepatic IL-24 expression along with increased protein levels of the autophagy receptors p62 and the autophagosome marker LC3-II (Fig. 1H; quantification in Fig. S1A), aligning with an inhibited autophagy-lysosome pathway [7]. This evidence concerns the gene SQSTM1 and metabolic dysfunction-associated steatohepatitis.