We evaluated the diagnostic performances of F‐CBA, RIPA and ELISA for anti‐AChR alone and combined with anti‐MuSK antibodies in MG diagnosis, the level of agreement among methods and the comparison of RIPA and ELISA anti‐AChR antibody concentration in generalised (GMG) and ocular (OMG) variants of MG. The gene discussed is MUSK; the disease is myasthenia gravis.