Similarly, using Doxycycline-inducible CRISPR interference (Dox-CRISPRi) to silence FRA1 in A375 (FRA1 high) and 1205Lu (FRA1 intermediate) human melanoma cells (Supplementary Fig. S1C) resulted in moderately decreased primary tumor growth when these cells were subcutaneously injected into NSG mice (Fig. 1C, Supplementary Fig. S1D). Here, FOSL1 is linked to melanoma.