Notably, MTHFR, AAK1, and MX1 expression levels were elevated in this high-risk group, suggesting their influence on sepsis prognosis potentially through modulation of immune and coagulation pathways (Supplementary Table 8).The diagnostic efficacy of these five genes was further validated in the external dataset GSE54514 (Supplementary Fig. 8). This evidence concerns the gene MTHFR and Sepsis.