Next, we examined CREB3L2 expression in HCC tissues at the protein level, 10 pairs of tissues were randomly selected from Cohort 1 for Western blotting, among the 9 pairs of samples, the expression level of CREB3L2 was higher in tumor tissues, with only one pair showing relatively lower expression in the tumor (Fig. 1E). The gene discussed is CREB3L2; the disease is hepatocellular carcinoma.