Furthermore, we treated HCC cells with cycloheximide(CHX) at various specific time points, our results demonstrated that the rate of degradation of SREBP1 protein was accelerated in 97H cells pretreated with CHX knockdown of CREB3L2, while the half-life of SREBP1 protein was extended in Huh7 cells overexpressing CREB3L2, compared to their respective controls, which reinforces that CREB3L2 can stabilize SREBP1 protein (Fig. 5E). This evidence concerns the gene SREBF1 and hepatocellular carcinoma.