To define the polyfunctionality of IL-18Rαhi and IL-18Rαlo subsets of influenza-specific DbNP366+ and DbPA224+ CD8 T cells, we first assessed the frequencies of IFNγ-producing CD8 T cells following NP366 or PA224 peptide stimulation in an intracellular cytokine secretion assay28. Here, CD8A is linked to influenza.