Naïve CD45.1+ OT-I cells (106) labelled with VPD450 were adoptively transferred into CD45.2+ C57BL/6 mice, then 24 hrs later mice were infected with 105 pfu A/HKx31-OVA and levels of IL-18Rα were determined in the draining mediastinal lymph node (mLN) at days 2.5, 3.5 and 4.5 after infection (Supplementary Fig. 10a). The gene discussed is IL18R1; the disease is infection.