As the genes encoding for the transcription factors Eomes and TCF1 (Tcf7), were differentially expressed in IL-18Rαlo and IL-18Rαhi OT-I cells in our RNASeq analysis (Fig. 5), we next sought to validate Eomes and TCF1 protein levels in influenza-specific DbNP366+ and DbPA224+ CD8 T cells following severe IAV infection (Fig. 6a, Supplementary Fig. 8c). This evidence concerns the gene EOMES and influenza.