METTL3 and pancreatic neoplasm: Subsequently, we evaluated the expression levels of IGF2BP3, METTL3 and m6A levels in several pancreatic cancer cell lines using Western blot and ELISA, The results showed that METTL3 and IGF2BP3 were mainly expressed in PANC-1 and AsPC-1 cells, compared to SW1990, BxPC-1, and Capan-1, and found a significant correlation between both METTL3 protein levels and m6A-modified mRNA levels and IGF2BP3 protein in pancreatic cancer cells (Fig. 2B, S1E).