IGF2BP3 and familial pancreatic carcinoma: We further demonstrated that silencing of IGF2BP3 reversed METTL3-induced upregulation of EMP1 in pancreatic cancer cells, and we also examined the mRNA and protein levels of EMP1 after knockdown of METTL3 in pancreatic cancer cells and found that it was not reversible after overexpression of IGF2BP3, demonstrating the role of the METTL3-IGF2BP3 axis in the regulation of EMP1 at the RNA and protein levels, as detected by qPCR and Western blotting (Fig. 3H, I, S1M).