DESI-MSI found increased levels of phospholipids in cancer cases, which were also supported by our RPPA results that showed overexpression of certain proteins (mTOR, B-Raf_pS445, MNK1, PAK1, b-catenin,) that are implicated in dysregulated endometrial cancer signaling pathways, such as the PI3K/Akt/mTOR, MAPK/RAS and Wnt pathways (Fig. 4). This evidence concerns the gene AKT1 and endometrial cancer.