To clarify the function of EMP1 and its role in MASLD-IRI pathogenesis, we used the UCSC CellBrowser single-cell sequencing database for bioinformatic analysis, and found that EMP1 is mainly expressed in liver non-parenchymal cells, including liver sinusoidal endothelial cells (LSECs), HSCs, and kupffer cells (KCs), but is mostly absent in hepatocytes (Fig. 3a,b). This evidence concerns the gene EMP1 and metabolic dysfunction-associated steatotic liver disease.