Cells: Primary BMMSCs.Animals: 3‐month‐old male wild type and Zmpste24−/− mice.Mode of disease induction: Genetically modification of mice, leading lacking the Zmpste24 gene to mimic age related osteoporosis.Treatment: 10−7 M Bay K8644 treatment (the potent L‐type Ca2+ channel agonist) for BMMSCs for 7 & 14 days & intraperitoneal injection of 1 mg/kg Bay K8644 for mice treatment for two months.Control: • Negative: treat with DMSO • Positive: no. Here, ZMPSTE24 is linked to osteoporosis.