Pamies et al. conducted the first study on human-derived NTera2/D1 (hNT2) embryonal carcinoma stem cells, confirming that the PNPLA6 gene plays a role in the early stages of neuro differentiation in vitro, with its primary biological impacting neurogenesis and epithelial tube morphogenesis (Pamies et al., 2014). This evidence concerns the gene PNPLA6 and embryonal carcinoma.