To assess the impact of myeloid NUDT21 depletion on ARDS, we treated Nudt21f/fLysmCre and age- and gender-matched LysmCre controls with LPS (2.5 mg/kg) via oropharyngeal aspiration (OPA) and analyzed the lungs 3 days post-treatment (Fig. 3B). Here, NUDT21 is linked to acute respiratory distress syndrome.