CHD3 and Glucose intolerance: Following confirmation of efficient recombination that included breeding in the Rosa26-Loxp-Stop-Loxp-tdTomato (R26LSL-tdTomato,17) lineage reporter (Supplementary Fig. 2c), we found that male Chd3/4Δβ mice displayed severe glucose intolerance and elevated ad libitum fed blood glucose, whereas female Chd3/4Δβ mice exhibited profound glucose intolerance without a significant increase in fed glycemia (Fig. 1a–d).