We also accessed the bar plot of USP5 gene expression profiles across all tumor samples and paired normal tissues leveraging the Gene Expression Profiling Interactive Analysis (GEPIA) database (Fig. 7B, C), uncovering its marked upregulation in 275 COAD tissues compared to 349 normal colon tissues, and its high expression in 92 READ tumor samples compared to 318 paired normal tissues, highlighting its potential role in colon carcinogenesis. This evidence concerns the gene USP5 and neoplasm.