USP5 and colon adenocarcinoma: To conduct a comprehensive analysis of USP5 expression in patients with colon adenocarcinoma (COAD) and rectum adenocarcinoma (READ), we first analyzed data from the Tumor Immune Estimation Resource (TIMER) database and determined that USP5 was highly expressed in COAD tumors (n = 457) compared with adjacent normal tissues (n = 41) and highly expressed in READ tumors (n = 166) compared with adjacent normal tissues (n = 10) (Fig. 7A).