To further reveal the potential mechanisms underlying ETV4-induced CRC metastasis, RNA sequencing (RNA-seq) analysis was performed to comprehensively examine the differential expression genes in stable ETV4 overexpression and control cells (Fig.3a), 6814 genes were up-regulated and 8036 genes were down-regulated in HCT116-ETV4 cells (|log2FC| ≥ 2.0) (Supplementary file 2). This evidence concerns the gene ETV4 and colorectal carcinoma.