To enhance ovarian TIL expansion and anti-tumor activity, we cultured TILs with single cells from fresh ovarian tumors under four different conditions (1):W (IL-2 alone), (2)WI (IL-2 + anti-PD1), (3)WIO (IL-2 + anti-PD1 + IL-15/21), and (4)WIOG (IL-2 + anti-PD1 + IL-15/21 + anti-GITR) (Figure 1A). Here, IL2 is linked to neoplasm.