ADGRE1 and neoplasm: Based on transcriptional similarity observed in UMAP space, distinct cell populations were manually identified and annotated using canonical lineage markers: tumor cells (Epcam+ Cdh1+), T/NK cells (Cd3d+, Nkg7+), B cells (Cd79a+), macrophages (Adgre1+, Cd68+), endothelial cells (Pecam1+), and fibroblasts (Pdgfrb+) (Figure 2B–C, Figure S1C).