Furthermore, the suppressive effect of ICB on shMap3k7-silenced tumors was completely abrogated when mice were pre-treated with both neutralizing anti-CD4 and anti-CD8 antibodies, but less with either alone (Fig. 7F, 7G), suggesting that both CD4+ and CD8+ T cells are essential for mounting a robust anti-tumor response. This evidence concerns the gene CD8A and neoplasm.