Furthermore, RNA sequencing comparing the transcriptional profiles of the dFLEx CDS tumors compared to sarcomas in KRASloxP-STOP-loxP-G12D p53fl/fl; (KP) mice, demonstrates dFLEx CDS tumors express known CIC::DUX4 target genes (14,21) such as Etv1/4/5, Dusp6, Shc3/4, and Spred3 (Figure 1E). Here, SPRED3 is linked to sarcoma.