MYCN and neuroblastoma: To elucidate the complexity of cellular compositions in NBs from primary and metastatic locations, we analyzed samples from patients with high-risk NB, diagnosed based on MYCN amplification (Fig. 1A and Supplementary Table S1), using single-cell multi-omics profiling including single-nucleus RNA-sequencing (snRNA-seq) and single-cell assay for transposase-accessible chromatin using sequencing (scATAC-seq).