Several genes that encode proteins involved in cancer invasion and related to EMT, such as IGFBP2, VGF, and SMYD3, were robustly expressed in metastasis-specific clusters, whereas adrenergic-related genes (CHGA, ROBO2) were more highly expressed in the differentiated cell state within NB tumor cell continuum (Fig. 1I and Supplementary Fig. S2B). This evidence concerns the gene CHGA and cancer.