In contrast to primary masses, the tumor states in NB metastases exhibited markedly upregulated networks associated with early neural development (e.g., TWIST1 and ASCL1 regulons in neuroblast progenitors), EMT and tumor dissemination (e.g., GATA638 and ZEB139 regulons in MES-Nbt1 cells and FOXN340 in MES-Nbt2 cells), and invasive oncogenic traits (e.g., TGIF2 in the DKK2+ Nbt-Met state) (Fig. 2I). This evidence concerns the gene TGIF2 and neuroblastoma.