VGF showed mild transcript-level decorrelation (0.57 → 0.53) with a marginal rise in RNA–protein correlation (0.30 → 0.31); the separation increased from D = 0.81 to D = 0.99, again moderate to moderate-to-strong, indicating coordinated reductions of VGF RNA and NPTX2 protein in AD (Fig. 3D and 3E). This evidence concerns the gene NPTX2 and Alzheimer disease.