Therefore, the upregulation of Ccn4 by exercise is not only a response to the activation of the Wnt pathway, but also likely constitutes a positive feedback loop itself, which further amplifies the osteogenic effect of exercise.In summary, under the state of obesity induced by a high-fat diet, factors such as chronic inflammation lead to the inhibition of the Wnt pathway and a reduction in bone formation. This evidence concerns the gene CCN4 and obesity due to melanocortin 4 receptor deficiency.