As our earlier results showed that HOXB13 KD had minimal impact on PC-3M cell proliferation, we generated intravenous PCa xenograft models by inoculating luciferase-labeled PC-3M cells with either control (pGIPZ) or HOXB13 KD into nude SCID mice through tail vein injection and monitored tumor metastasis. This evidence concerns the gene HOXB13 and posterior cortical atrophy.