TSC2 and cancer: Nine cell lines with mTORC1 hyperactivation secondary to TSC1/TSC2 loss from various organs/tissues (6 from human cancers) were exposed to 500 nM rapamycin for more than 3 months, leading to generation of RP cells (Supplemental Table 1; supplemental material available online with this article; https://doi.org/10.1172/jci.insight.187448DS1).