We aimed to evaluate the performance of a semiquantitative test for G6PD deficiency, the SD Biosensor Standard G6PD Test with the Brewer's method, in a reference site for the treatment of infectious diseases.<h4>Method</h4>We evaluate the diagnostic accuracy performance of the SD Biosensor Standard G6PD Test in 125 individuals with infectious diseases and other illnesses.<h4>Main results</h4>We observed a trend of concordance between the G6PD status (Deficient or Normal), with low frequencies of discordances in the screenings. The gene discussed is G6PD; the disease is G6PD deficiency.