In the three GBM cell lines and murine GBM GL261 cells, we assessed CLIC1 expression levels, tmCLIC1 translocation, and measured its ion conductance in CRISPR-Cas9 negative control (NC), Clic1−/−, and rescued cells (Supplementary Fig. 1), confirming the efficacy of gene deletion and rescue. This evidence concerns the gene CLIC1 and glioblastoma.